Blackburn climbed out the bottom three by beating Newcastle on Matchday 18, enabling Brighton to move within two points of the summit.
Bolton Wanderers were the only team in the top four to win on Matchday 20, moving into second and closing the gap on Scunthorpe United.
Carlisle United are the new Sky Bet League Two leaders, following their victory over Mansfield, and Plymouth's third successive loss on Matchday 19.
It was a record-breaking EFL Cup Round Four, with an all-time high attendance level and some thriling on-field action.
With a new format for Season 2016/17, 64 teams will be aiming to follow in Barnsley’s footsteps from 2015 and be victorious at Wembley in April.
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