Huddersfield Town hit the summit on Matchday 4, whilst Leeds moved away from the relegation places with their first win.
Bolton Wanderers continued their perfect start by winning at home to Fleetwood on Matchday 4, which moved them two clear of Scunthorpe and Bradford.
Morecambe's win at Yeovil took them to the top of the table on Matchday 4, and Exeter moved off the bottom after they picked up their first victory.
The highlights of a thrilling Round Two saw Accrington, Northampton and Gillingham beat top-flight opposition in Burnley, West Brom and Watford.
With a new format for Season 2016/17, 64 teams will be aiming to follow in Barnsley’s footsteps from 2015 and be victorious at Wembley in April.
Get our newsletter