Brighton's Friday night win over Leeds ensured they topped the standings ahead of Matchday 20.
Bolton Wanderers were the only team in the top four to win on Matchday 20, moving into second and closing the gap on Scunthorpe United.
Doncaster Rovers moved to the Sky Bet League Two summit on Matchday 19, while Yeovil Town beat Crawley 5-0 to go sixth.
It was a record-breaking EFL Cup Round Four, with an all-time high attendance level and some thriling on-field action.
With a new format for Season 2016/17, 64 teams will be aiming to follow in Barnsley’s footsteps from 2015 and be victorious at Wembley in April.
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