Wolverhampton Wanderers U21s required penalties to get past Sunderland U21s in the first Round Two game, following a 1-1 draw across 90 minutes.Nine other ties were played 24 hours later (Tuesday 6th December), with another three fixtures needing spot-kicks to decide them.
Blackpool edged Doncaster Rovers 8-7 in a shoot-out, while Sky Bet League One leaders Scunthorpe United got past Morecambe 5-3, and Oxford United edged Southend United 4-3 - all after drawing 1-1 in normal time.
Yeovil Town were the only side to claim a home victory across 90 minutes on Tuesday, when they came from behind to beat Milton Keynes Dons 4-1, and compound misery on Robbie Neilson in his first game in charge of the visitors.
That meant five wins on the road elsewhere. In an early kick-off, Swansea City U21s left Norwich City U21s with a 1-0 victory to ease the long journey back to South Wales, while Luton Town prevailed 3-2 in a see-saw game against Swindon Town at the County Ground.
Mansfield Town also secured a narrow 3-2 result at Carlisle United, the side that put five past them in Sky Bet League Two 10 days earlier, and Brighton & Hove Albion U21s and Chesterfield beat AFC Wimbledon and Rochdale respectively.
On Wednesday evening (7th December) there was no need for shoot-outs, as Bradford City and Coventry City picked 1-0 home wins to put out Cambridge United and Crawley Town, while Wycombe Wanderers travelled to Millwall and were 3-1 victors.
Six days later, Oldham Athletic came from behind on the road to knock out Walsall, and book a Round Three tie with Mansfield Town.
In the week approaching Christmas, Reading U21s and Southampton U21s went head-to-head and, with the scores level after 90 minutes, the Royals were 4-3 victors on penalties.
Draw: Click here to see the Checkatrade Trophy Round Three draw
Checkatrade Trophy: Round Two results
Monday 5th December 2016
Wolverhampton Wanderers U21 1-1 Sunderland U21 (Wolves won 4-3 on pens)
Tuesday 6th December 2016
AFC Wimbledon 1-2 Brighton & Hove Albion U21
Carlisle United 2-3 Mansfield Town
Doncaster Rovers 1-1 Blackpool (Blackpool won 8-7 on penalties)
Norwich City U21 0-1 Swansea City U21
Rochdale 0-2 Chesterfield
Scunthorpe United 1-1 Morecambe (Scunthorpe won 5-3 on penalties)
Southend United 1-1 Oxford United (Oxford won 4-3 on penalties)
Swindon Town 2-3 Luton Town
Yeovil Town 4-1 Milton Keynes Dons
Wednesday 7th December 2016
Bradford City 1-0 Cambridge United
Coventry City 1-0 Crawley Town
Millwall 1-3 Wycombe Wanderers
Tuesday 13th December 2016
Walsall 1-3 Oldham Athletic
Wednesday 21st December 2016
Southampton U21 1-1 Reading U21 (Reading won 4-3 on penalties)
Tuesday 10th January 2017
Cheltenham Town v Leicester City U21