Shrewsbury Town clinched promotion to Sky Bet League 1 with a 1-0 victory away at Cheltenham Town.It meant Town, along with Tranmere Rovers, dropped out of the Football League as Hartlepool United sealed their Sky Bet League 2 safety in the penultimate round of fixtures.
Managerless Rovers slipped into non-league football for the first time in 94 years following a 3-2 loss to Plymouth Argyle, while Gary Johnson's Robins will join them in the fifth tier next season after going down 1-0 to second-placed Shrewsbury, who celebrated promotion thanks to Jean-Louis Akpa Akpro's strike.
Hartlepool's Jordan Hugill, a former Tranmere loanee and brought to the north east by ex-Rovers boss Ronnie Moore, scored Pools' winner in a 2-1 success over Exeter City after Grecians striker Graham Cummins had cancelled out Scott Fenwick's opener.
Burton Albion remain top of the table after their 3-1 victory over Northampton Town, with Lucas Akins scoring a brace and Phil Edwards also on target for the Brewers and Lawson D'Ath grabbing the Cobblers' consolation.
The race for the final top-three spot will see Southend United head into the final day two points ahead of Bury and three ahead of Wycombe Wanderers as Phil Brown's side moved into third.
The Shrimpers kept an eighth clean sheet and recorded a seventh straight win, despite losing Ben Coker to a red card, as Michael Timlin earned them a 1-0 triumph over Luton Town.
Gareth Ainsworth's Chairboys dropped two places as a Paul Mullin strike gave Morecambe an away success, while goals from Tom Soares and Ryan Lowe moved Bury up to fourth following a 2-0 success over AFC Wimbledon at the JD Stadium.
Morecambe are Southend's opponents next Saturday while Bury visit already-relegated Tranmere and Wycombe travel to Northampton.
Stevenage booked their Play-Off berth as Dean Parrett scored the only goal in a home success over Carlisle United, and seventh-placed Plymouth Argyle will almost certainly join them in the end-of-season knockouts after extending their advantage over Luton to three points.
Ross Reid, Lewis Alessandra and Tareiq Holmes-Dennis all found the net for the Pilgrims as efforts from Max Power and Kayode Odejayi for Tranmere proved to be in vain.
Jamie Cureton, Matt Partridge, Frankie Raymond and Jodi Jones were all on target as Dagenham & Redbridge thrashed Accrington 4-0, while Kemar Roofe and Danny Hylton netted as Oxford United beat Cambridge United 2-0.
Portsmouth picked up their first three points of the post-Andy Awford era by beating Mansfield Town 2-1. The Stags were down to 10 men as Ritchie Sutton was sent off after 78 seconds and Matt Tubbs and Jed Wallace scored in either half for Pompey, with Jack Thomas pulling one back.
Finally, York City's last home game of the campaign ended in a 2-0 loss to Newport County as David Tutonda and Lee Minshull added to their accounts in the second half.