Millwall boss Kenny Jackett accused referee Gavin Ward of costing his side a valuable point in their bid to steer clear of the relegation zone as they went down 2-1 at home to Blackburn.
The Lions and Blackburn looked set to settle for a point apiece until Ward adjudged Danny Shittu had tripped Grant Hanley in the area.
Millwall accused Hanley of a dive and implored Ward to consult his assistant. But the decision stood and Jordan Rhodes stepped up to score from the spot and secure a win for the visitors.
Jackett said: "That decision has cost us. I've just seen it again and it looked like a soft decision, in my opinion the referee got it wrong, it didn't look like there was any contact.
"I was looking for the refereeing team to make the right call as it looked soft at the time, and having seen it again it was."
Shittu's protests at the final whistle earned him a red card and Jackett added: "Danny's very upset but I have to back my player. He's a passionate man and he felt very passionately that there was no contact. Unfortunately it was a second yellow which we cannot appeal."
Rovers caretaker boss Gary Bowyer had not seen the incident again but said: "I thought it was a great run from Hanley, he deserves credit for that, and from where I stood I jumped up because I thought it was a penalty.
"I don't think we should be talking about that, we should be talking about our second-half performance and how brave my players were. Some you get, some you don't."
Rovers certainly improved after the break, having fallen behind to Karleigh Osborne's first goal for Millwall. The defender finished like a striker when he lashed the ball home from eight yards after Rovers failed to clear James Henry's free-kick.
They levelled with a freak goal in the 56th minute when David Jones' corner deceived Rob Hulse on the near post, and goalkeeper David Forde, and squirmed over the line. And 12 minutes from time Rhodes kept his head while all about were losing theirs, coolly sending Forde the wrong way from the spot for his 27th goal of the season.