Newcastle United went top on Matchday 26, as Brighton's unbeaten streak came to an end at Preston.
Sheffield United remain top, but Scunthorpe United have cut the gap to a single point.
Doncaster and Plymouth extended their advantage over Carlisle, while Luton are into the Play-Off positions.
It was a record-breaking EFL Cup Round Four, with an all-time high attendance level and some thriling on-field action.
With a new format for Season 2016/17, 64 teams will be aiming to follow in Barnsley’s footsteps from 2015 and be victorious at Wembley in April.
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